In vitro antiproliferative effect of aqueous extract of Solanum macranthum fruits on MDA-MB-231 tripple negative breast cancer cell line

The current investigation was focused on the assessment of antiproliferative effects of the Solanum macranthumaqueous fruits extract against the human breast cancer cell line MDA-MB-231 in vitro. Phytochemical analysisof the S. macranthum aqueous fruits extract was performed and the phytoconstituents found positive foralkaloids, phenols, and saponins. Quantitative analysis of the total alkaloid and phenol was determined usinggravimetric analysis and folins-phenols reagent method, respectively. The total alkaloid present was estimatedto be 7.58% and total phenol of 158.774 mg/Gallic Acid Equivalent. The cytotoxicity of S. macranthumaqueous fruits extract was screened using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide(MTT) assay and camptothecin was used as standard reference drug. At the concentration of 500 µg/ml, theaqueous extract potentially inhibited the growth of the MDA-MB-231 cell line in vitro reaching 19%, and theIC50 value was calculated to be 525.59 μg/ml. Thus, indicating that aqueous extract of the S. macranthum fruitspossess potential anticancer property.

Bidirectional Screening of Tuberculosis Patients for Diabetes Mellitus and Diabetes Patients for Tuberculosis

To assess the feasibility and results of screening diabetes mellitus (DM) patients for tuberculosis (TB) and TB patients for DM within the routine health care setting. Prospective observational study carried out within the Diabetes Centre and Pulmonary Medicine Department from February 2012 to September 2012. The screening for active TB in DM and DM in TB patients is followed as per the guidelines of the Revised National Tuberculosis Control Programme and national programmes in India. Total of 307 patients diagnosed with TB during the study period. Among the TB patients 9.77% were smokers, 19.54% were known cases diabetes, and 15.96% were newly diagnosed cases of diabetes. Total of 4,118 diabetes patients were screened for TB in which 111 patients found to have TB. The strengths of this study are that we implemented screening within the routine health system. It is feasible to screen DM patients for TB resulting in high rates of TB detection.